Novel HA-PVA/NOCC bilayered scaffold for osteochondral tissue-engineering applications – Fabrication, characterization, in vitro and in vivo biocompatibility study
نویسندگان
چکیده
This study aimed to examine the bilayered scaffold containing hydroxyapatite (HA) and N, Ocarboxymethylated chitosan (PVA/NOCC) seeded with mesenchymal stem cells in vitro to determine its potential use as a scaffold in tissue engineering. SEM analysis revealed that pores in this scaffold were interconnected with parallel grooves and ridges. EDS indicated that the level of calcium was relatively higher in HA. FTIR confirmed the presence of functional groups corresponding to alkyl halide, alkyne, hydroxyl group, and alkane groups, while XRD results revealed that the phase content of HA and hydrogel comprised organic substance. Significantly higher levels of differentiation markers, namely, alkaline phosphatase and glycosaminoglycans, were detected in the scaffold cultures. Furthermore, histology and blood profile demonstrated biocompatibility of this bilayered scaffold in a rat model. In conclusion, our results demonstrate that bilayered scaffold may be a promising construct for osteochondral defects. & 2013 Elsevier B.V. All rights reserved.
منابع مشابه
Biocompatibility and Toxicity of Poly(vinyl alcohol)/N,O-Carboxymethyl Chitosan Scaffold
The in vivo biocompatibility and toxicity of PVA/NOCC scaffold were tested by comparing them with those of a biocompatible inert material HAM in a rat model. On Day 5, changes in the blood parameters of the PVA/NOCC-implanted rats were significantly higher than those of the control. The levels of potassium, creatinine, total protein, A/G, hemoglobulin, erythrocytes, WBC, and platelets were not ...
متن کاملIn vitro and in vivo studies of a novel bacterial cellulose-based acellular bilayer nanocomposite scaffold for the repair of osteochondral defects
Bacterial cellulose (BC) is a naturally occurring nanofibrous biomaterial which exhibits unique physical properties and is amenable to chemical modifications. To explore whether this versatile material can be used in the treatment of osteochondral defects (OCD), we developed and characterized novel BC-based nanocomposite scaffolds, for example, BC-hydroxyapatite (BC-HA) and BC-glycosaminoglycan...
متن کاملA novel fabrication of PVA/Alginate-Bioglass electrospun for biomedical engineering application
Objecttive (s): Polyvinylalcohol (PVA) is among the most natural polymers which have interesting properties such as nontoxic nature, biodegradability and high resistance to bacterial attacks making it applicable for tissue scaffolds, protective clothing, and wound healing.Materials and Methods: In the current work, PVA and Na-Alginate nanocomposite scaffolds were prepared using the electrospinn...
متن کاملN-O, carboxymethyl chitosan enhanced scaffold porosity and biocompatibility under e-beam irradiation at 50 kGy.
In this study, a chitosan co-polymer scaffold was prepared by mixing poly(vinyl alcohol) (PVA), NO, carboxymethyl chitosan (NOCC) and polyethylene glycol (PEG) solutions to obtain desirable properties for chondrocyte cultivation. Electron beam (e-beam) radiation was used to physically cross-link these polymers at different doses (30 kGy and 50 kGy). The co-polymers were then lyophilized to form...
متن کاملNovel hydroxyapatite/chitosan bilayered scaffold for osteochondral tissue-engineering applications: Scaffold design and its performance when seeded with goat bone marrow stromal cells.
Recent studies suggest that bone marrow stromal cells are a potential source of osteoblasts and chondrocytes and can be used to regenerate damaged tissues using a tissue-engineering (TE) approach. However, these strategies require the use of an appropriate scaffold architecture that can support the formation de novo of either bone and cartilage tissue, or both, as in the case of osteochondral d...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2013